Sunday, July 09, 2017

Gelam Honey Helps Treat Eye Injuries

The Potential of Gelam Honey in Promoting the Proliferative Phase of Corneal Reepithelialization

Wounds. 2017 Jun 28


The aim of this study is to investigate the potential benefits of Gelam honey (GH) in promoting proliferation of ex vivo cor-neal epithelial cells (CECs) and its effects on the phenotypical features.


Corneal epithelial cells were isolated from the corneas of rabbits (n = 6). The optimal dose of GH for CEC proliferation in both basal medium (BM) and cornea medium (CM) was determined via MTT (3-[4, 5-dimethyl thiazolyl-2]-2, 5-diphenyl tetrazolium bro- mide) assay. Morphology, gene and protein expressions, and cell cycle analysis of CECs were evaluated via phase contrast microscopy, real- time polymerase chain reaction, immunocytochemistry, and ow cytometry, respectively.


Corneal epithelial cells cultured in 0.0015% GH-supplemented media (BM + 0.0015% GH; CM + 0.0015% GH) demonstrated optimal proliferative capacity with normal polygonal- shaped morphology. Gelam honey potentiates cytokeratin 3 (CK3) gene expression in accordance with the cytoplasmic CK3 protein expression while retaining normal cell cycle of CECs.


Culture media treated with 0.0015% GH increased CEC proliferation while preserving its phenotypical features. This study demonstrated the potential development of GH-based topical treatment for superficial corneal injury.

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